Dual Barcode Indices for Multiplex Sequencing with In-Solution Protein Arrays

Description:

With the continued advancement of new methods to stratify samples and diseases, there is an increased demand for high throughput (HTP) tools to detect and quantify multiple variables simultaneously. While there are numerous tools that enable HPT analyses of the genome, similar methods and tools for proteomic analyses are not available or reliable. Although the genome is a great blue print, it does not always reflect the actual state of biology at any time. Thus, HTP tools that more closely reflect the current state of biology, such as proteomic analyses, are needed.

 

Researchers at the Biodesign Institute of Arizona State University, originally developed novel assays, using multiple affinity reagents in a multiplexed set, to measure many different specific protein targets quantitatively and simultaneously. Several different approaches had been developed to measure the target proteins, to increase versatility of the assays. More recently, they’ve further developed the assays by incorporating an additional set of barcodes into the samples, such that now multiplexed analysis of samples of interest can be achieved. Thousands of proteins, antigens and samples of interest can be measured simultaneously with an extraordinary dynamic range.

 

The quantitative measurement of thousands of proteins, or any detectable antigens, as well as thousands of samples, simultaneously, in a very high throughput manner, saves time, reduces associated costs and is a game changer for proteomic analyses.

 

Potential Applications

•       High throughput proteome measurements

o       Can measure thousands of proteins/antigens simultaneously

o       Can measure thousands of samples simultaneously/in one run

 

Benefits and Advantages

•       Can be multiplexed in the thousands to save time and reduce costs

•       Thousands of samples of interest can be combined and the results determined in a single NGS run

•       The affinity reagents can be chosen by the user for enhanced flexibility and tailorability

•       Uses standard or tailored affinity reagents for increased flexibility

o       The affinity reagents detect proteins with a certain conformation

o       The affinity reagents detect proteins with a specific modification

•       Does not require the user to remain within a closed system, such as a proprietary set of affinity reagents

•       Wide dynamic range

•       Multiple techniques for measuring the abundance of the target       

o       Increased versatility

 

Related Intellectual Property: 

US 9,938,523 B2

US 10,717,977 B2

US 10,618,932 B2

For more information about the inventor(s) and their research, please see

Dr. LaBaer's departmental webpage

Dr. Park's departmental webpage  

 

Case ID:
M20-179L^
Published:
04-26-2021
Last Updated:
04-26-2021

For More Information, Contact