Methods and Systems for Continuous Production of Dehalococcoides

Description:

Researchers at the Biodesign Institute of Arizona State University have developed a method and system for continuous production of Dehalococcoides in continuous flow stirred-tank reactors (CSTRs). Through optimization of growth conditions, growth medium, and reactor construction materials, a density of 10¹² cells/L of Dehalococcoides was achieved in just three days, which is a tenfold improvement over the best reported culture process.

This method provides the fastest production rate of high-cell density Dehalococcoides cultures and has the potential to revolutionize production of bioaugmentation cultures for treatment of chlorinated ethenes.

Potential Applications

• High density production of Dehalococcoides in continuous flow stirred-tank reactors

Benefits and Advantages

• Rapid, higher density production - only three days to reach a cell density of 10¹² cells/L
• Cultures can be stored for longer durations - helps streamline production
• The novel growth medium minimizes proliferation of competing microorganisms and enhances growth of beneficial microbes
• These Dehalococcoides cultures convert trichloroethene (TCE) to mostly ethene at a 3-d HRT
• The reactor construction materials minimize possible inhibition of anaerobic microorganisms in the CSTR

For more information about the inventor(s) and their research, please see
Dr. Krajmalnik-Brown's directory webpage