Single-cell In Situ DNA and/or RNA Sequencing
Description:
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Single cell DNA and/or RNA sequencing
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Cancer evolution – tumor pathogenicity, drug resistance, transcriptional profiling of tumor diversity, etc.
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Microbiome diversity
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Transcriptome diversity – particularly in mutant strains/libraries
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Developmental genetics – gametocyte characterization
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Plant genetics – understanding genes that contribute to drought or temperature tolerance
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Highly scalable - allows simultaneous multiplexing of single cells from up to 96 different growth conditions or genetic backgrounds
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Can identify rare or low frequency mutations in a population
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Provides a more detailed picture of microbes in specific environments
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Can characterize cells that all have unique DNA assortment, such as gametocytes
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Can determine the distribution of heterogeneous genomes in a population of cells, such as a tumor
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Captures genetic diversity broadly or at a region of interest while simultaneously sampling the transcriptome
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User-friendly - does not require cell sorting or isolation
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Each whole genome is amplified and tagged with barcodes inside the original cell
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Conflicting reagents can be washed without interfering with the genetic material within the cell – multiple chemical and/or enzymatic reactions can be applied
- Case ID:
- M21-300LC^
- Published:
- 05-26-2022
- Last Updated:
- 06-20-2022
Categories
Bioanalytical Assays, Chemistries & Devices Genomic Assays/Reagents/Tools Life Science (All LS Techs)For More Information, Contact
- Jovan Heusser
- jheusser@asu.edu